ABSTRACT
In this approach, a new voltammetric method for determination of norfloxacin was proposed with high sensitivity and wider detection linear range. The used voltammetric sensor was fabricated simply by coating a layer of graphene oxide (GO) and Nafion composited film on glassy carbon electrode. The advantage of proposed method was sensitive electrochemical response for norfloxacin, which was attributed to the excellent electrical conductivity of GO and the accumulating function of Nafion under optimum experimental conditions, the present method revealed a good linear response for determination of norfloxacin in the range of 1×10-8mol/L-7×10-6 mol/L with a detection limit of 5×10-9 mol/L. The proposed method was successfully applied in the determination of norfloxacin in capsules with satisfactory results...
Propos-se, por essa abordagem, novo método voltamétrico, com alta sensibilidade e faixa linear de detecção mais ampla, para a determinação de norfloxacino. O sensor voltamétrico utilizado foi fabricado simplismente por cobertura de camada de óxido de grafeno (GO) e filme de Nafion em eletrodo de cabrono vítreo. A vantagem do método proposto foi a resposta eletroquímica sensível para o norfloxacino, atribuída à condutividade elétrica excelente do GO e à função acumulada do Nafion. Sob condições experimentais ótimas, o presente método revelou boa resposta linear para a determinação do norfloxacino na faixa de limite de detecção de 1×10-8mol/L-7×10-6 mol/L. O método proposto foi aplicado com sucesso na determinação de norfloxacino em cápsulas, com resultados satisfatórios...
Subject(s)
Humans , Norfloxacin/analysis , Chemistry, Pharmaceutical/methods , Electrochemical Techniques/instrumentation , Electrochemical Techniques/methodsABSTRACT
Aims: To evaluate the effect of Dimethyl sulfoxide (DMSO) on multiple myeloma (MM) cells. Study Design: Experimental study. Place and Duration of Study: Department of Pathology and Genomic Medicine, the Methodist Hospital, Cancer Pathology Lab, the Methodist Hospital Research Institute, between 2011 and 2013. Methodology: We treated RPMI 8226 and Dox-40 MM cells with DMSO. The cell growth, proliferation, apoptosis, and colony formation were examined. Results: Exposure of RPMI 8226 and Dox-40 myeloma cells to low concentrations of DMSO resulted in a marked increase in cell growth as detected by viable cell counts and cell proliferation analysis. This DMSO-stimulated cell growth showed a dose-dependent pattern and could reach a maximal 3.57 fold-increase in the presence of 0.2% DMSO. In contrast, other common solvents including methanol and ethanol had little or no effect on cell growth. In addition, the in vitro cell transformation assay by colony formation in soft agar culture revealed that the presence of low concentrations of DMSO significantly enhanced potential of oncogenesis of myeloma cells. Conclusion: Taken together, the findings demonstrate that DMSO could stimulate growth and the in vitro transformation of myeloma cells. However, further work is needed to understand the effect of DMSO on the pathogenesis and progression of MM.
ABSTRACT
The expression of octamer binding factor 4 (Oct4) gene in bladder cancer cell line T24 and its effects on the biological characteristics of the cells were investigated.RT-PCR and Western blot were employed to detect the expression of Oct4 in T24 cells.The changes of biological charac-teristics in T24 cells were analyzed before and after gene-silencing by Boyden chamber and MTT.The results showed that the expression of Oct4 gene was detectable in T24 cells by RT-PCR and Western blot.The expression of Oct4 gene and protein was down-regulated by siRNA,and average number of transwell cells in interference group,negative control group and blank control group was 101.40±4.56,104.20±10.03 and 111.00±11.90,respectively.There was significant difference in the proliferation ability of the cells from 48 h,72 h to 96 h after the interference by siRNA between in-terference group and negative group or blank control group (P<0.05).It was suggested that Oct4 gene was related with proliferation ability ofT24 cells,but not with invasive capability.